使用基于嗜热栖热菌的高度链特异性逆转录PCR检测庚型肝炎病毒复制位点
Detection of hepatitis G virus replication sites by using highly strand-specific Tth-based reverse transcriptase PCR.
作者信息
Laskus T, Radkowski M, Wang L F, Vargas H, Rakela J
机构信息
Division of Transplantation Medicine, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pennsylvania 15213, USA.
出版信息
J Virol. 1998 Apr;72(4):3072-5. doi: 10.1128/JVI.72.4.3072-3075.1998.
Abstract
The replication sites of the recently discovered hepatitis G virus (HGV) remain unknown. Using highly strand-specific Tth-based reverse transcriptase PCR, we searched for the presence of viral RNA negative strand in multiple autopsy tissues from four patients with AIDS and in peripheral blood mononuclear cells from six other human immunodeficiency virus-positive patients. Negative-strand HGV RNA was detected in three of four bone marrow samples, in two of two spleen samples, and in one of four liver tissue samples. However, the specific cellular site of replication within the positive tissues was not determined. This study does not support HGV as a primary hepatotropic virus.
摘要
最近发现的庚型肝炎病毒（HGV）的复制位点尚不清楚。我们使用高度链特异性的基于Tth的逆转录酶PCR，在4例艾滋病患者的多个尸检组织以及另外6例人类免疫缺陷病毒阳性患者的外周血单核细胞中寻找病毒RNA负链的存在。在4份骨髓样本中的3份、2份脾脏样本中的2份以及4份肝组织样本中的1份中检测到了HGV RNA负链。然而，阳性组织内具体的复制细胞位点尚未确定。本研究不支持HGV是一种主要嗜肝病毒的观点。
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