Laskus T, Radkowski M, Wang L F, Cianciara J, Vargas H, Rakela J
Division of Transplantation Medicine, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, PA 15213, USA.
J Gen Virol. 1997 Nov;78 ( Pt 11):2747-50. doi: 10.1099/0022-1317-78-11-2747.
Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.
采用基于Tth的链特异性逆转录聚合酶链反应（RT-PCR），对27例丙型肝炎病毒（HCV）感染患者的外周血单个核细胞（PBMC）进行分析，以检测HCV负链RNA的存在情况。在任何样本中均未检测到负链RNA，且从PBMC中扩增出的正链HCV序列与血清中发现的序列相同。这些结果表明，HCV不在PBMC中复制，该部位HCV序列的存在与被动病毒吸附和/或循环病毒污染相符。
