维罗纳气单胞菌温和生物变种中金属β-内酰胺酶ImiS的核苷酸和氨基酸序列
Nucleotide and amino acid sequences of the metallo-beta-lactamase, ImiS, from Aeromonas veronii bv. sobria.
作者信息
Walsh T R, Neville W A, Haran M H, Tolson D, Payne D J, Bateson J H, MacGowan A P, Bennett P M
机构信息
Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, United Kingdom.
出版信息
Antimicrob Agents Chemother. 1998 Feb;42(2):436-9. doi: 10.1128/AAC.42.2.436.
Abstract
The Aeromonas veronii bv. sobria metallo-beta-lactamase gene, imiS, was cloned. The imiS open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pI of 8.1. To confirm the predicted sequence, purified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Both possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta-lactamases.
摘要
维罗纳气单胞菌温和生物变种的金属β-内酰胺酶基因imiS被克隆。imiS开放阅读框延伸762 bp，编码一个含254个氨基酸的蛋白质，其分泌型修饰蛋白含227个氨基酸，预测等电点为8.1。为确认预测序列，对纯化的ImiS进行酶切并鉴定所得肽段，结果显示ImiS序列与预测一致，与CphA的序列一致性为98%。两者在88至92位均具有推定的活性位点序列Asn-Tyr-His-Thr-Asp，这是气单胞菌金属β-内酰胺酶所特有的。
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