UL97磷酸转移酶中Cys607突变为Tyr的变化，使从两名免疫功能低下患者体内分离出的两株人巨细胞病毒对更昔洛韦产生耐药性。
The Cys607-->Tyr change in the UL97 phosphotransferase confers ganciclovir resistance to two human cytomegalovirus strains recovered from two immunocompromised patients.
作者信息
Baldanti F, Underwood M R, Talarico C L, Simoncini L, Sarasini A, Biron K K, Gerna G
机构信息
Servizio di Virologia, IRCCS Policlinico S. Matteo, Pavia, Italy.
出版信息
Antimicrob Agents Chemother. 1998 Feb;42(2):444-6. doi: 10.1128/AAC.42.2.444.
Abstract
Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains recovered from an AIDS patient (strain VR4990) and a heart transplant recipient (strain VR5474) showed a Cys607-->Tyr change in the UL97-encoded phosphotransferase. No amino acid substitutions were observed in the viral DNA polymerase. Marker transfer experiments showed marked reduction in GCV phosphorylation and drug susceptibility of the recombinant HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involved in GCV substrate recognition.
摘要
从一名艾滋病患者（VR4990株）和一名心脏移植受者（VR5474株）体内分离出的两株耐更昔洛韦（GCV）的人巨细胞病毒（HCMV），其UL97编码的磷酸转移酶发生了Cys607→Tyr变化。在病毒DNA聚合酶中未观察到氨基酸替换。标记转移实验表明，重组HCMV株VR4990rec2-1-1的GCV磷酸化和药物敏感性显著降低。这些结果进一步扩展了参与GCV底物识别的UL97磷酸转移酶羧基末端结构域的区域。
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Evolution of mutations conferring multidrug resistance during prophylaxis and therapy for cytomegalovirus disease.巨细胞病毒疾病预防和治疗期间赋予多重耐药性的突变的演变
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