Rowbottom A W, Lepper M W, Sharpstone D, Gazzard B
Department of Pathology and Microbiology, University of Bristol, UK.
Clin Exp Immunol. 1998 Mar;111(3):559-63. doi: 10.1046/j.1365-2249.1998.00515.x.
HIV+ individuals with human CMV (HCMV) reactivation have a CD3 receptor-mediated T cell hyporesponsiveness when compared with CD4-matched HIV+ and HCMV- control groups. The impairment of proliferation was not reversed by exogenous IL-2. A typical increase in NFkappaB expression was observed following cross-linking of the CD3 receptor, but did not lead to increased CD25 cell surface expression or cell proliferation. The HCMV-induced non-responsiveness was not observed when cells were stimulated with phorbol esters. Lymphocytes cultured with media collected from cell cultures infected with HCMV showed a dose-dependent inhibition in the total T cell population even though cells staining dually for CD8/57 increased in number. The altered growth factor requirements of CD8/57+ cells may therefore account for their presence in AIDS and patients following bone marrow transplantation.
与CD4匹配的HIV阳性且人巨细胞病毒（HCMV）阴性的对照组相比，HIV阳性且伴有HCMV再激活的个体存在CD3受体介导的T细胞低反应性。外源性白细胞介素-2并不能逆转增殖障碍。CD3受体交联后可观察到核因子κB（NFκB）表达典型性增加，但这并未导致CD25细胞表面表达增加或细胞增殖。当用佛波酯刺激细胞时，未观察到HCMV诱导的无反应性。用感染HCMV的细胞培养物收集的培养基培养淋巴细胞，即使CD8/57双染细胞数量增加，总T细胞群体也呈现剂量依赖性抑制。因此，CD8/57+细胞生长因子需求的改变可能解释了它们在艾滋病患者和骨髓移植患者中的存在。
