Bryant N J, Stevens T H
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
Microbiol Mol Biol Rev. 1998 Mar;62(1):230-47. doi: 10.1128/MMBR.62.1.230-247.1998.
Delivery of proteins to the vacuole of the yeast Saccharomyces cerevisiae provides an excellent model system in which to study vacuole and lysosome biogenesis and membrane traffic. This organelle receives proteins from a number of different routes, including proteins sorted away from the secretory pathway at the Golgi apparatus and endocytic traffic arising from the plasma membrane. Genetic analysis has revealed at least 60 genes involved in vacuolar protein sorting, numerous components of a novel cytoplasm-to-vacuole transport pathway, and a large number of proteins required for autophagy. Cell biological and biochemical studies have provided important molecular insights into the various protein delivery pathways to the yeast vacuole. This review describes the various pathways to the vacuole and illustrates how they are related to one another in the vacuolar network of S. cerevisiae.
将蛋白质递送至酿酒酵母的液泡提供了一个出色的模型系统，可用于研究液泡和溶酶体的生物发生以及膜运输。这个细胞器从许多不同途径接收蛋白质，包括在高尔基体处从分泌途径分选出来的蛋白质以及源自质膜的内吞运输。遗传分析揭示了至少60个参与液泡蛋白分选的基因、一种新型细胞质到液泡运输途径的众多组分以及自噬所需的大量蛋白质。细胞生物学和生物化学研究为酵母液泡的各种蛋白质递送途径提供了重要的分子见解。本综述描述了通向液泡的各种途径，并说明了它们在酿酒酵母的液泡网络中是如何相互关联的。
