Horín P, Cermák P, Vojtísek P, Vinkler I
Institute of Animal Breeding and Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Zentralbl Veterinarmed B. 1998 Feb;45(1):25-9. doi: 10.1111/j.1439-0450.1998.tb00762.x.
Acid treatment of bovine lymphocytes by a buffered solution of 0.263 m citric acid and 0.123 M Na2HPO4 at pH = 3.0, originally described for human and murine lymphocytes, selectively eliminated the antigenicity of MHC (BoLA) class I determinants also from bovine lymphocytes. The viability of acid-treated cell suspension was higher than 90%. The reactivity of acid-treated lymphocytes with BoLA class I typing alloantisera was lost in microcytotoxicity test, while their reactions with cross-reactive anti-HLA class II, anti-BoCD2 and BoCD5 monoclonal antibodies, and with antisera detecting two non-MHC lymphocyte alloantigenic specificities (BoLY w1 and R') remained unchanged in the microcytotoxicity and/or indirect immunofluorescence tests. The results thus show that this approach of modulating cell surface expression of MHC class I determinants may be used in cattle.
最初用于人和鼠淋巴细胞的、由pH = 3.0的0.263 m柠檬酸和0.123 M磷酸氢二钠缓冲溶液对牛淋巴细胞进行酸处理，也能选择性地消除牛淋巴细胞中MHC（BoLA）I类决定簇的抗原性。酸处理后的细胞悬液活力高于90%。在微量细胞毒性试验中，酸处理的淋巴细胞与BoLA I类分型同种抗血清的反应性丧失，而在微量细胞毒性和/或间接免疫荧光试验中，它们与交叉反应性抗HLA II类、抗BoCD2和BoCD5单克隆抗体以及检测两种非MHC淋巴细胞同种抗原特异性（BoLY w1和R'）的抗血清的反应保持不变。因此，结果表明这种调节MHC I类决定簇细胞表面表达的方法可用于牛。
