Kruk Z L, Cheeta S, Milla J, Muscat R, Williams J E, Willner P
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
J Neurosci Methods. 1998 Jan 31;79(1):9-19. doi: 10.1016/s0165-0270(97)00156-8.
Fast cyclic voltammetry (FCV) was used to measure real time release of electrically stimulated endogenous dopamine in the nucleus accumbens (NAc) of conscious freely moving rats for up to 17 days. The method of electrode construction, implantation, electrical stimulation and recording of changes of extracellular dopamine concentration in the conscious rat are described. Rats trained on a continuous reinforcement schedule to perform intracranial self stimulation (ICSS) were implanted with electrodes for FCV. During ICSS, no faradaic signal was observed at an electrode implanted in the NAc. Decreasing the intensity of the stimulating current abolished ICSS, increasing the stimulating current disrupted ICSS. Operator delivered electrical stimulations using currents greater than those needed for ICSS yielded dopamine signals. It is concluded that during ICSS, sufficient dopamine does not reach the extracellular fluid space to yield a faradaic signal detectable by FCV.
快速循环伏安法（FCV）用于测量清醒自由活动大鼠伏隔核（NAc）中电刺激内源性多巴胺的实时释放，最长可达17天。描述了清醒大鼠电极构建、植入、电刺激以及细胞外多巴胺浓度变化记录的方法。在连续强化训练计划下进行颅内自我刺激（ICSS）训练的大鼠植入用于FCV的电极。在ICSS期间，植入NAc的电极未观察到法拉第信号。降低刺激电流强度可消除ICSS，增加刺激电流会干扰ICSS。实验者使用大于ICSS所需电流进行电刺激时会产生多巴胺信号。得出的结论是，在ICSS期间，没有足够的多巴胺到达细胞外液空间以产生FCV可检测到的法拉第信号。
