Kampf G, Höfer M, Rüden H
Institut für Hygiene, Freie Universität Berlin.
Zentralbl Hyg Umweltmed. 1998 Feb;200(5-6):457-64.
An insufficient neutralization of chlorhexidine (4%), tested in vitro against Enterococcus faecium ATCC 6057, was suspected in the quantitative suspension test although the combination Tween 80, cysteine, histidine and saponine was assessed as sufficient for neutralising chlorhexidine. Bactericidal activity of each neutralising compound and their combinations was excluded. Sufficient neutralization was observed when all dilution steps and the corresponding agar plates were supplemented with the neutralising compounds. Successful neutralization in broth is not equivalent with successful neutralization in tryptic saline. We suggest to supplement at least the agar plates of the first dilution step with neutralising compounds when testing chlorhexidine.
在针对粪肠球菌ATCC 6057进行的体外定量悬液试验中，怀疑对4%洗必泰的中和作用不足，尽管评估吐温80、半胱氨酸、组氨酸和皂角苷的组合足以中和洗必泰。排除了每种中和化合物及其组合的杀菌活性。当所有稀释步骤和相应的琼脂平板都补充了中和化合物时，观察到了充分的中和作用。肉汤中的成功中和与胰蛋白胨盐水中的成功中和并不等同。我们建议在测试洗必泰时，至少在第一个稀释步骤的琼脂平板中补充中和化合物。
