Nichols J R, Schingoethe D J, Maiga H A, Brouk M J, Piepenbrink M S
Dairy Science Department, South Dakota State University, Brookings 57007-0647, USA.
J Dairy Sci. 1998 Feb;81(2):482-91. doi: 10.3168/jds.S0022-0302(98)75600-0.
Twelve multiparous Holstein cows averaging 57 d (36 to 77 d) postpartum at the start of the experiment were utilized in a replicated 4 x 4 Latin square design. Dietary protein supplements were 1) soybean meal, 2) soybean meal plus ruminally protected Lys and Met, 3) corn distillers grains, and 4) corn distillers grains plus ruminally protected Lys and Met. Dry matter intakes were lower for cows fed diets containing soybean meal than for cows fed diets containing corn distillers grains. Milk yield increased with the corn distillers grains (34.3, 34.0, 35.3, and 36.7 kg/d for cows fed diets 1 through 4, respectively), especially when supplemented with ruminally protected Lys and Met. Milk protein yield and percentage were increased by amino acid supplementation. Milk fat yield and percentage were unaffected by diet. The only milk protein fraction affected was nonprotein N, which was lower in the milk of cows fed corn distillers grains. Lysine, Met, and Phe were indicated as the most limiting amino acids for all diets when using amino acid extraction efficiency and transfer efficiency to indicate limiting amino acids. When corn distillers grains were supplemented with ruminally protected Lys and Met, milk yield and milk protein yield and percentage increased because the diet containing corn distillers grains was probably deficient in Lys.
12头经产荷斯坦奶牛在试验开始时平均产后57天(36至77天),采用重复4×4拉丁方设计。日粮蛋白质补充剂分别为:1)豆粕;2)豆粕加瘤胃保护性赖氨酸和蛋氨酸;3)玉米酒糟;4)玉米酒糟加瘤胃保护性赖氨酸和蛋氨酸。采食含豆粕日粮的奶牛干物质采食量低于采食含玉米酒糟日粮的奶牛。玉米酒糟组牛奶产量增加(采食日粮1至4的奶牛分别为34.3、34.0、35.3和36.7千克/天),尤其是添加瘤胃保护性赖氨酸和蛋氨酸时。氨基酸补充提高了牛奶蛋白质产量和百分比。牛奶脂肪产量和百分比不受日粮影响。唯一受影响的牛奶蛋白质组分是非蛋白氮,采食玉米酒糟日粮的奶牛牛奶中该组分含量较低。当使用氨基酸提取效率和转移效率来表明限制性氨基酸时,赖氨酸、蛋氨酸和苯丙氨酸被表明是所有日粮中最具限制性的氨基酸。当玉米酒糟添加瘤胃保护性赖氨酸和蛋氨酸时,牛奶产量、牛奶蛋白质产量和百分比均增加,因为含玉米酒糟的日粮可能缺乏赖氨酸。