Lesser S S, Sherwood N T, Lo D C
Department of Neurobiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Mol Cell Neurosci. 1997;10(3-4):173-83. doi: 10.1006/mcne.1997.0656.
Neurotrophic factors profoundly affect neuronal differentiation, but whether they influence neuronal phenotype in instructive ways remains unclear: do different neurotrophic factors always trigger identical programs of differentiation or can each impose distinct functional properties even when acting upon the same population of target neurons? We addressed this issue by examining the regulatory effects of the four neurotrophins on the molecular components of electrical excitability, voltage-gated ion channels, within a single cellular context. Using patch clamp methods, we studied neurotrophin regulation of voltage-gated sodium, calcium, and potassium currents in SK-N-SH neuroblastoma cells. We found that each neurotrophin induced a unique pattern of expression of ionic currents despite similar activation of initial signal transduction events. Thus, each neurotrophin imposed a different excitable phenotype even when acting upon the same target cells.
神经营养因子对神经元分化有深远影响，但它们是否以指导性方式影响神经元表型仍不清楚：不同的神经营养因子总是触发相同的分化程序，还是即使作用于同一群靶神经元时，每种神经营养因子也能赋予不同的功能特性？我们通过在单一细胞环境中研究四种神经营养蛋白对电兴奋性分子成分（电压门控离子通道）的调节作用来解决这个问题。使用膜片钳方法，我们研究了神经营养蛋白对SK-N-SH神经母细胞瘤细胞中电压门控钠、钙和钾电流的调节。我们发现，尽管初始信号转导事件的激活相似，但每种神经营养蛋白都诱导了独特的离子电流表达模式。因此，即使作用于相同的靶细胞，每种神经营养蛋白也会赋予不同的可兴奋表型。
