Sirivan P, Obayashi M, Nakamura M, Tantaswasdi U, Takehara K
National Institute of Animal Health, Chatuchak, Bangkok, Thailand.
Avian Dis. 1998 Jan-Mar;42(1):133-9.
By using primers (AL18F2 and AL18R2) designed from goose parvovirus (GPV) strain IHC, an 806-bp band was amplified by polymerase chain reaction (PCR) from all of 17 samples from Thailand. Specificity to GPV was confirmed by Southern hybridization. With restriction enzyme digestion of the PCR products, two isolates differed from the other 15 isolates by the absence of restriction sites for HincII and BglII and the presence of EcoR1 site. Nucleotide sequence analysis of the PCR products from the different groups revealed that one group is GPV and the other group is Muscovy duck parvovirus (MDPV). Thus restriction enzyme fragment length polymorphism analysis of the PCR products could be used to distinguish GPV and MDPV. The data showed that GPV and MDPV are present in Thailand.
通过使用从鹅细小病毒（GPV）IHC株设计的引物（AL18F2和AL18R2），通过聚合酶链反应（PCR）从泰国的所有17个样本中扩增出一条806bp的条带。通过Southern杂交确认了对GPV的特异性。对PCR产物进行限制性酶切，两个分离株与其他15个分离株的不同之处在于，它们没有HincII和BglII的限制性位点，而有EcoR1位点。对不同组的PCR产物进行核苷酸序列分析表明，一组是GPV，另一组是番鸭细小病毒（MDPV）。因此，PCR产物的限制性酶切片段长度多态性分析可用于区分GPV和MDPV。数据表明泰国存在GPV和MDPV。
