Saito H, Kanamori Y, Takemori T, Nariuchi H, Kubota E, Takahashi-Iwanaga H, Iwanaga T, Ishikawa H
Department of Microbiology, Keio University School of Medicine, Tokyo 160, Japan.
Science. 1998 Apr 10;280(5361):275-8. doi: 10.1126/science.280.5361.275.
Cryptopatches (CPs) are part of the murine intestinal immune compartment. Cells isolated from CPs of the small intestine that were c-kit positive (c-kit+) but lineage markers negative (Lin-) gave rise to T cell receptor (TCR) alphabeta and TCR gammadelta intestinal intraepithelial T cells after in vivo transfer or tissue engraftment into severe combined immunodeficient mice. In contrast, cells from Peyer's patches and mesenteric lymph nodes, which belong in the same intestinal immune compartment but lack c-kit+Lin- cells, failed to do so. These findings and results of electron microscopic analysis provide evidence of a local intestinal T cell precursor that develops in the CPs.
隐窝斑块（CPs）是小鼠肠道免疫区室的一部分。从小肠CPs中分离出的细胞，其c-kit阳性（c-kit+）但谱系标志物阴性（Lin-），在体内转移或组织移植到严重联合免疫缺陷小鼠后，可产生T细胞受体（TCR）αβ和TCRγδ肠道上皮内T细胞。相比之下，来自派尔集合淋巴结和肠系膜淋巴结的细胞，它们属于相同的肠道免疫区室，但缺乏c-kit+Lin-细胞，则无法产生这些细胞。这些发现以及电子显微镜分析结果为在CPs中发育的局部肠道T细胞前体提供了证据。
