Rege R V, Prystowsky J B
Department of Surgery, Chicago Health Care System, Lakeside Division, Illinois, USA.
J Surg Res. 1998 Jan;74(1):81-5. doi: 10.1006/jsre.1997.5213.
Based on previous work which suggested that biliary crystals may induce inflammation in the gallbladder wall and that inflammation is an early event during the formation of pigment gallstones in the dog, studies were performed examining mucus layer thickness, myeloperoxidase activity, and interleukin-1 (IL-1) activity in the wall of mouse gallbladder during formation and growth of cholesterol gallstones.
The inflammatory effects of cholesterol gallstones at 2 and 4 weeks were studied in BalB/C mice fed a crushed standard mouse chow with added cholesterol (1.0%) and cholic acid (0.5%). Results were compared to those of normal mice fed standard mouse chow. The presence or absence of crystals and stones was determined by gross and microscopic examination of bile. Myeloperoxidase and IL-1 activity in the gallbladder wall was measured using well-established bioassays. Mucus layer thickness was measured by darkfield microscopy.
All mice fed a lithogenic, 1.0% cholesterol/0.5% cholic acid diet developed cholesterol crystals and gallstones at 2 and 6 weeks. No control mice developed either crystals or gallstones. Myeloperoxidase and IL-1 activities, markers of an inflammatory response, increased significantly in the gallbladder of mice with crystals at 2 weeks. Myeloperoxidase activity increased two- to three-fold, and IL-1 activity sevenfold, by 6 weeks. Mucus layer thickness also progressively increased during the 6-week period.
It is concluded that inflammation is an early event associated with the appearance of crystals and gallstones in bile.
基于先前的研究工作，这些研究表明胆汁晶体可能诱发胆囊壁炎症，且炎症是犬类色素胆结石形成过程中的早期事件，因此开展了相关研究，以检测胆固醇胆结石形成和生长过程中小鼠胆囊壁的黏液层厚度、髓过氧化物酶活性和白细胞介素-1（IL-1）活性。
在喂食添加了胆固醇（1.0%）和胆酸（0.5%）的碾碎标准小鼠饲料的BalB/C小鼠中，研究了2周和4周时胆固醇胆结石的炎症效应。将结果与喂食标准小鼠饲料的正常小鼠的结果进行比较。通过对胆汁进行大体和显微镜检查来确定晶体和结石的存在与否。使用成熟的生物测定法测量胆囊壁中的髓过氧化物酶和IL-1活性。通过暗视野显微镜测量黏液层厚度。
所有喂食致石性1.0%胆固醇/0.5%胆酸饮食的小鼠在2周和6周时均形成了胆固醇晶体和胆结石。没有对照小鼠形成晶体或胆结石。炎症反应标志物髓过氧化物酶和IL-1活性在2周时晶体形成的小鼠胆囊中显著增加。到6周时，髓过氧化物酶活性增加了两到三倍，IL-1活性增加了七倍。在6周期间，黏液层厚度也逐渐增加。
得出的结论是，炎症是与胆汁中晶体和胆结石出现相关的早期事件。
