Bevan A, Burns G F, Gray L, Cawley J C
Department of Haematological Medicine, University of Cambridge, England.
Scand J Immunol. 1980;11(2):223-33. doi: 10.1111/j.1365-3083.1980.tb00229.x.
Acid phosphatase and esterase cytochemistry performed on purified normal human T-cell populations showed that both methods produced distinctive localized dot patterns of reactivity in 60-70% of cells. By examination of rosette preparations formed with ox erythrocytes coated with IgM (EAM), with IgG (EAG), or anti-human kappa and lambda light chains, it was shown that this pattern of reactivity was largely restricted to small T lymphocytes possessing receptors for the Fc of IgM (T mu cells). In addition, both B lymphocytes and T cells with receptors for the Fc of IgG (T gamma cells) were larger lymphocytes with more abundant cytoplasm and usually displayed scattered granular acid phosphatase activity; in esterase preparations both cell types were either negative or possessed similar scattered granular positivity. As compared with T mu cells, T gamma cells were seen to form loose spontaneous rosettes with sheep erythrocytes. Combined esterase and acid phosphatase staining showed that both enzyme activities in the T mu cells are localized in the same area, and ultrastructural acid phosphatase cytochemistry established that this was in distinctive lysosomal structures. T mu staining by both esterase and acid phosphatase cytochemistry was greatly reduced after rosetting with EAG, but not after rosette formation with EAM or sheep erythrocytes.
对纯化的正常人T细胞群体进行酸性磷酸酶和酯酶细胞化学分析表明，两种方法在60%-70%的细胞中均产生了独特的局灶性点状反应模式。通过检查用IgM包被的牛红细胞（EAM）、IgG包被的牛红细胞（EAG）或抗人κ和λ轻链形成的玫瑰花结制剂，发现这种反应模式主要局限于具有IgM Fc受体的小T淋巴细胞（Tμ细胞）。此外，B淋巴细胞和具有IgG Fc受体的T细胞（Tγ细胞）都是较大的淋巴细胞，细胞质更丰富，通常表现出散在的颗粒状酸性磷酸酶活性；在酯酶制剂中，这两种细胞类型要么呈阴性，要么具有相似的散在颗粒状阳性。与Tμ细胞相比，Tγ细胞可与绵羊红细胞形成松散的自发玫瑰花结。联合酯酶和酸性磷酸酶染色表明，Tμ细胞中的两种酶活性都定位于同一区域，超微结构酸性磷酸酶细胞化学证实这是在独特的溶酶体结构中。用EAG进行玫瑰花结形成后，酯酶和酸性磷酸酶细胞化学对Tμ细胞的染色大大减少，但用EAM或绵羊红细胞形成玫瑰花结后则没有减少。
