Freitas-Fernandes L B, Rundegren J, Arnebrant T, Glantz P O
Department of Periodontology, Faculty of Odontology, Lund University, Malmö, Sweden.
Acta Odontol Scand. 1998 Feb;56(1):2-8. doi: 10.1080/000163598422983.
Gel electrophoresis was used to analyze precipitates formed of delmopinol hydrochloride or chlorhexidine digluconate mixed with unstimulated whole saliva samples from five test subjects. Final concentrations of delmopinol (6.4 mM) or chlorhexidine (6.4 mM, 2.2 mM) mixed with whole saliva were incubated for 10 min at 37 degrees C. The precipitates were pelleted by centrifugation and resuspended to a similar protein density. The protein patterns in the pellets were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, using 12.3% gels. The amount of pellet protein was determined by densitometry in four molecular weight ranges (10-21.5, 21.5-26, 26-45, and 45-300). The results indicated that high molecular weight (45-300) proteins dominated in the precipitate and that 2.2 mM chlorlhexidine precipitated more salivary protein than 6.4 mM. At equimolar concentration (6.4 mM) delmopinol precipitated more high molecular weight salivary proteins than chlorhexidine.
采用凝胶电泳法分析盐酸地莫匹醇或葡萄糖酸氯己定与五名受试对象未刺激的全唾液样本混合形成的沉淀物。将与全唾液混合的地莫匹醇（6.4 mM）或氯己定（6.4 mM、2.2 mM）的终浓度在37℃下孵育10分钟。通过离心使沉淀物沉淀并重悬至相似的蛋白质密度。使用12.3%的凝胶，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析沉淀物中的蛋白质模式。通过光密度测定法在四个分子量范围（10 - 21.5、21.5 - 26、26 - 45和45 - 300）中测定沉淀蛋白质的量。结果表明，沉淀物中高分子量（45 - 300）蛋白质占主导，并且2.2 mM氯己定比6.4 mM沉淀出更多的唾液蛋白质。在等摩尔浓度（6.4 mM）下，地莫匹醇比氯己定沉淀出更多的高分子量唾液蛋白质。
