Holmes A L, Raper R N, Heilig J S
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.
Genetics. 1998 Mar;148(3):1189-201. doi: 10.1093/genetics/148.3.1189.
To identify genes necessary for establishing connections in the Drosophila sensory nervous system, we designed a screen for mutations affecting development of the larval visual system. The larval visual system has a simple and stereotypic morphology, can be recognized histologically by a variety of techniques, and is unnecessary for viability. Therefore, it provides an opportunity to identify genes involved in all stages of development of a simple, specific neuronal connection. By direct observation of the larval visual system in mutant embryos, we identified 24 mutations affecting its development; 13 of these are larval visual system-specific. These 13 mutations can be grouped phenotypically into five classes based on their effects on location, path or morphology of the larval visual system nerves and organs. These mutants and phenotypic classifications provide a context for further analysis of neuronal development, pathfinding and target recognition.
为了鉴定在果蝇感觉神经系统中建立连接所必需的基因，我们设计了一个筛选影响幼虫视觉系统发育的突变体的实验。幼虫视觉系统具有简单且刻板的形态，可以通过多种组织学技术识别，并且对于生存能力并非必需。因此，它提供了一个机会来鉴定参与简单、特定神经元连接发育各个阶段的基因。通过直接观察突变胚胎中的幼虫视觉系统，我们鉴定出24个影响其发育的突变；其中13个是幼虫视觉系统特异性的。基于它们对幼虫视觉系统神经和器官的位置、路径或形态的影响，这13个突变在表型上可分为五类。这些突变体和表型分类为进一步分析神经元发育、路径寻找和靶标识别提供了背景。
