Luo Y, Raible D, Raper J A
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia 19104.
Cell. 1993 Oct 22;75(2):217-27. doi: 10.1016/0092-8674(93)80064-l.
Repulsive guidance cues can steer neuronal growth cones during development and prevent mature axons from regenerating. We have identified a 100 kd glycoprotein in the chick brain that is a good candidate for a repulsive cue. Since it induces the collapse and paralysis of neuronal growth cones in vitro, we have named it collapsin. It is effective at concentrations of approximately 10 pM. The C-terminal half of collapsin contains a single immunoglobulin-like domain and an additional highly basic region. The N-terminal half of collapsin shares significant homology with fasciclin IV, a growth cone guidance protein in grasshopper. Recombinant collapsin causes sensory ganglion growth cones to collapse but not retinal ganglion cell growth cones. We propose that collapsin could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism.
排斥性导向因子在发育过程中可引导神经元生长锥，并阻止成熟轴突再生。我们在鸡脑中鉴定出一种100kd的糖蛋白，它很可能是一种排斥性信号分子。由于它在体外可诱导神经元生长锥的塌陷和麻痹，我们将其命名为坍塌素。它在浓度约为10 pM时有效。坍塌素的C端含有一个免疫球蛋白样结构域和一个额外的高碱性区域。坍塌素的N端与草蜢中的一种生长锥导向蛋白——成束蛋白IV有显著的同源性。重组坍塌素可导致感觉神经节生长锥塌陷，但不会使视网膜神经节细胞生长锥塌陷。我们认为，坍塌素可以作为一种配体，通过抑制运动的机制来引导特定的生长锥。
