Kocher T D, Lee W J, Sobolewska H, Penman D, McAndrew B
Department of Zoology, University of New Hampshire, Durham 03824, USA.
Genetics. 1998 Mar;148(3):1225-32. doi: 10.1093/genetics/148.3.1225.
We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of approximately 1000-1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes.
我们利用DNA标记构建了尼罗罗非鱼的遗传图谱。在来自单一雌性的41个单倍体胚胎中研究了62个微卫星标记和112个匿名片段长度多态性（AFLP）的分离情况。我们确定了这些标记中162个（93.1%）之间的连锁关系。在最终图谱中，95%的微卫星标记和92%的AFLP标记是连锁的。该图谱跨越704个科桑比厘摩，分布在30个连锁群中，覆盖了该物种的22条染色体。其中24个连锁群至少包含一个微卫星多态性标记。根据相距15厘摩或更少厘摩标记的数量，我们估计图谱总长度约为1000 - 1200厘摩。观察到高水平的干扰，这与其他鱼类的测量结果一致。该图谱是丽鱼科鱼类单基因座和数量性状定位的起点。
