Sagawa H, Tatsumi N
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
Osaka City Med J. 1997 Dec;43(2):199-208.
Proflavine, an acridine analog for industrial use, was used to stain blood cells. A drop of blood treated with ethylenediaminetetraacetic acid-2K was mixed with a 0.00001% solution of the dye and observed immediately by fluorescence microscopy with a green filter. Leukocytes, platelets, and reticulocytes were stained but mature red blood cells were not. Chromatin in the nuclei of all leukocytes and nucleoli of lymphocytes and monocytes had greenish-yellow fluorescence, and the kind of cell could be identified by the tone and intensity of this color. Granules in granulocytes were in green. Reticular fine-granular or granulofibrous structures in the reticulocytes were brownish. The proflavine could be used routinely in clinical laboratories because this single stain makes possible simultaneous differentiation of leukocytes and counting of reticulocytes.
吖啶黄，一种工业用吖啶类似物，被用于对血细胞进行染色。一滴用乙二胺四乙酸二钾处理过的血液与0.00001%的该染料溶液混合，立即使用配备绿色滤光片的荧光显微镜进行观察。白细胞、血小板和网织红细胞被染色，但成熟红细胞未被染色。所有白细胞细胞核中的染色质以及淋巴细胞和单核细胞核仁发出绿黄色荧光，并且可以通过这种颜色的色调和强度来识别细胞类型。粒细胞中的颗粒呈绿色。网织红细胞中的网状细颗粒或颗粒纤维结构呈褐色。吖啶黄可在临床实验室常规使用，因为这种单一染色使得白细胞的同时鉴别和网织红细胞计数成为可能。
