Roy A, Ghosh A K
Department of Applied Biochemistry, Indian Institute of Chemical Biology, Calcutta, India.
Biochim Biophys Acta. 1998 Mar 5;1401(3):235-8. doi: 10.1016/s0167-4889(97)00156-0.
The levels of two trehalose hydrolysing enzymes, acid trehalase (AT) and neutral trehalase (NT), have been investigated in Candida utilis at different stages of growth; in complete contrast to Saccharomyces cerevisiae, significant AT activity appears to be absent at all stages of growth studied in C. utilis. In addition, presence of only very low amounts of iso-aspartyl methyl transferase (IMT) activity at the onset of stationary phase and lower survival ability in early stationary phase in contrast to that of S. cerevisiae lend support to the ideas that (a) lower degree of survival of C. utilis in the stationary phase may be a direct consequence of inability to mobilise stored trehalose due to absence of intracellular AT and reduced levels of IMT activities and (b) trehalose may have a dual role vis-à-vis stress resistance in yeasts.
已对产朊假丝酵母在不同生长阶段的两种海藻糖水解酶——酸性海藻糖酶（AT）和中性海藻糖酶（NT）的水平进行了研究；与酿酒酵母形成鲜明对比的是，在所研究的产朊假丝酵母的所有生长阶段似乎都不存在显著的AT活性。此外，与酿酒酵母相比，在稳定期开始时仅存在极少量的异天冬氨酰甲基转移酶（IMT）活性，且在稳定早期的生存能力较低，这支持了以下观点：（a）产朊假丝酵母在稳定期较低的存活率可能是由于缺乏细胞内AT以及IMT活性水平降低而无法动员储存的海藻糖的直接后果；（b）海藻糖在酵母抗逆性方面可能具有双重作用。
