Hanks C T, Fang D, Sun Z, Edwards C A, Butler W T
University of Michigan, Ann Arbor 48109-1078, USA.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:260-6. doi: 10.1111/j.1600-0722.1998.tb02185.x.
Only four established odontoblast-like cell lines have been reported in the literature (1-6). Of the four, only two synthesize dentin-specific proteins. These studies report that the cell line MO6-G3 synthesizes phosphophoryn (DPP), dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1) while MDPC-23 synthesizes DSP, but not DMP-1. The objective of the present study was to determine whether polyclonal antibodies to rat DSP and DPP would label odontoblasts on microscopic sections of day-19 fetal mouse incisor odontoblasts as well as cultured cells of the MDPC-23 cell line. The spontaneously immortalized MDPC-23 cell line was derived from fetal mouse molar papillae, made continuous by the 3T6 method and cloned by dilution. These cultures have been passaged 77 times after cloning, form multilayered nodules, and have high alkaline phosphatase activity. The data show positive reactivity in odontoblasts in 19-d mouse fetal incisors as well as in cultures of MDPC-23 cells by fluorescence and confocal microscopy. In addition, these cultures were characterized by phase microscopy and scanning and transmission electron microscopy. These findings suggest that MDPC-23 cells are of the odontoblast lineage.
文献中仅报道了4种已建立的成牙本质细胞样细胞系(1 - 6)。在这4种细胞系中，只有2种能合成牙本质特异性蛋白。这些研究报告称，MO6 - G3细胞系能合成磷蛋白（DPP）、牙本质涎蛋白（DSP）和牙本质基质蛋白-1（DMP - 1），而MDPC - 23细胞系能合成DSP，但不能合成DMP - 1。本研究的目的是确定针对大鼠DSP和DPP的多克隆抗体是否能标记第19天胎鼠切牙成牙本质细胞的显微切片以及MDPC - 23细胞系的培养细胞。自发永生化的MDPC - 23细胞系源自胎鼠磨牙乳头，通过3T6方法使其连续传代，并通过稀释法克隆。这些培养物在克隆后已传代77次，形成多层结节，且具有高碱性磷酸酶活性。通过荧光显微镜和共聚焦显微镜观察，数据显示在19天龄小鼠胎儿切牙的成牙本质细胞以及MDPC - 23细胞培养物中存在阳性反应。此外，通过相差显微镜、扫描电子显微镜和透射电子显微镜对这些培养物进行了表征。这些发现表明MDPC - 23细胞属于成牙本质细胞谱系。
