Tanaka K, Xie M, Yanagi Y
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Arch Virol. 1998;143(2):213-25. doi: 10.1007/s007050050281.
Measles virus (MV) is efficiently isolated from patients with measles by using B95a cells, a marmoset B cell line. Recent wild-type MV strains isolated using B95a cells did not produce cytopathic effects in any of CD46+ primate cell lines examined (except B95a cells), nor did they induce downregulation of CD46. Transfection of the hemagglutinin (H) and fusion (F) genes of the Edmonston strain of MV produced syncytia in HeLa, Cos and B95a cells. By contrast, the expression of the H gene from the two wild-type strains, together with the F gene of the Edmonston strain, resulted in syncytium production in B95a cells, but not in HeLa and Cos cells. Cocultivation of Cos cells expressing the wild-type H protein and the Edmonston strain F protein with B95a cells, but not with HeLa, Jurkat or BJAB cells, generated large syncytia. The results suggest that these recent MV isolates may use a molecule other than CD46 as the cellular receptor or require another coreceptor to infect cells.
通过使用狨猴B细胞系B95a细胞，可从麻疹患者中有效分离出麻疹病毒（MV）。最近使用B95a细胞分离出的野生型MV毒株，在任何检测的CD46 +灵长类细胞系（B95a细胞除外）中均未产生细胞病变效应，也未诱导CD46下调。MV埃姆斯毒株的血凝素（H）和融合（F）基因转染后，可在HeLa、Cos和B95a细胞中产生多核巨细胞。相比之下，两种野生型毒株的H基因与埃姆斯毒株的F基因共同表达，可在B95a细胞中产生多核巨细胞，但在HeLa和Cos细胞中则不会。将表达野生型H蛋白和埃姆斯毒株F蛋白的Cos细胞与B95a细胞共培养可产生大型多核巨细胞，而与HeLa、Jurkat或BJAB细胞共培养则不会。结果表明，这些最近分离出的MV毒株可能使用CD46以外的分子作为细胞受体，或者需要另一种共受体来感染细胞。
