Ascoli V, Mastroianni C M, Galati V, Sirianni M C, Fruscalzo A, Pistilli A, Lo Coco F
Dipartimento di Medicina Sperimentale e Patologia, Università La Sapienza, Rome, Italy.
Haematologica. 1998 Jan;83(1):8-12.
Primary effusion lymphomas (PELs) containing Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8/HHV-8) DNA sequences represent a distinct but heterogeneous group of rare non-Hodgkin's lymphomas of null-cell phenotype/B-cell origin. We aimed to describe the clinicopathologic features of two human immunodeficiency virus (HIV)-related PELs occurring in homosexual men with Kaposi's sarcoma (KS).
Thoracentesis was followed by morphologic plus immunophenotypic studies and molecular analysis of tumor cell DNA by means of combination of polymerase chain reaction and Southern blot analysis.
Patients developed recurrent lymphomatous effusions lacking tissue involvement, in the context of severe immunodepression (CD4 count < 60/microL) and anti-retroviral therapy. The effusions disclosed an immunoblast-like population CD45/CD30+, but B-cell- and T-cell-associated antigen negative, showing clonal immunoglobulin heavy chain gene rearrangements and harbouring HHV-8 DNA sequences. One case contained Epstein-Barr virus genome with no evidence of c-myc, bcl-2 and bcl-6 gene alterations. Both patients had aggressive disease.
These cases represent additional examples of PEL associated with HHV-8 and confirm that the group of HIV-positive homosexual men may be at highest risk for PEL.
含有卡波西肉瘤相关疱疹病毒（人类疱疹病毒8型/HHV - 8）DNA序列的原发性渗出性淋巴瘤（PEL）是一组独特但异质性的罕见非霍奇金淋巴瘤，起源于无细胞表型/B细胞。我们旨在描述在患有卡波西肉瘤（KS）的同性恋男性中发生的两例人类免疫缺陷病毒（HIV）相关PEL的临床病理特征。
进行胸腔穿刺术，随后进行形态学及免疫表型研究，并通过聚合酶链反应和Southern印迹分析相结合的方法对肿瘤细胞DNA进行分子分析。
患者在严重免疫抑制（CD4计数<60/μL）和抗逆转录病毒治疗的背景下，出现复发性淋巴瘤性渗出液，无组织受累。渗出液中发现免疫母细胞样细胞群，CD45/CD30阳性，但B细胞和T细胞相关抗原阴性，显示克隆性免疫球蛋白重链基因重排，并含有HHV - 8 DNA序列。其中一例含有爱泼斯坦 - 巴尔病毒基因组，无c - myc、bcl - 2和bcl - 6基因改变的证据。两名患者均患有侵袭性疾病。
这些病例是与HHV - 8相关的PEL的更多实例，并证实HIV阳性同性恋男性群体可能是PEL的最高风险人群。
