有丝分裂原激活的人淋巴细胞的趋化作用及膜活性酶的影响。
Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes.
作者信息
Wilkinson P C, Roberts J A, Russell R J, McLoughlin M
出版信息
Clin Exp Immunol. 1976 Aug;25(2):280-7.
Following incubation with polyclonal activators such as PHA or concanavalin A, human peripheral blood lymphocytes show directional migration towards chemotactic factors such as endotoxin-activated plasma, casein or denatured proteins. This migration is inhibited by treatment of the cells with phospholipase C and sphingomyelinase C, but little affected by proteases or glycosidases. In addition, these lymphocytes migrate towards PHA and other lymphocyte activators when these reagents are used as chemoattractants at concentrations well below their mitogenic doses. They also migrate towards staphylococcal protein A. Migration towards PHA and protein A is reduced by pretreating the lymphocytes with proteases but not with phospholipase C. These results suggest two independent membrane interactions which initiate lymphocyte chemotaxis, one acting directly via the lipid bilayer, the other involving the binding of ligands to membrane proteins.
在用多克隆激活剂如PHA或伴刀豆球蛋白A孵育后，人外周血淋巴细胞会朝着趋化因子如内毒素激活的血浆、酪蛋白或变性蛋白进行定向迁移。用磷脂酶C和鞘磷脂酶C处理细胞会抑制这种迁移，但蛋白酶或糖苷酶对其影响很小。此外，当这些试剂以远低于其促有丝分裂剂量的浓度用作趋化剂时，这些淋巴细胞会朝着PHA和其他淋巴细胞激活剂迁移。它们也会朝着葡萄球菌蛋白A迁移。用蛋白酶预处理淋巴细胞会降低其对PHA和蛋白A的迁移，但用磷脂酶C预处理则不会。这些结果表明存在两种独立的膜相互作用启动淋巴细胞趋化作用，一种直接通过脂质双层起作用，另一种涉及配体与膜蛋白的结合。
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