Wilkinson P C
Immunology. 1977 Sep;33(3):407-12.
The binding of antibody-coated chicken erythrocytes (EA) to human blood lymphocytes and monocytes is inhibited by pretreatment of the leucocytes with sphingomyelinase C. Inhibition of rosetting of neuraminidase-treated EA with neutrophils is also seen with this enzyme. The cholesterol-binding theta-toxin of Clostridium perfringens and pronase also inhibit EA-rosette formation, but less strongly than sphingomyelinase. The lipid-specific agents also inhibit chemotactic migration of leucocytes to casein and denatured HSA, whereas proteases and glycosidases do not. These results suggest that membrane lipids are important constituents of the binding sites for Fc fragments and for certain chemotactic factors and point to an important role for sphingomyelin in this binding.
用鞘磷脂酶C预处理白细胞可抑制抗体包被的鸡红细胞（EA）与人血淋巴细胞及单核细胞的结合。该酶也能抑制经神经氨酸酶处理的EA与中性粒细胞的玫瑰花结形成。产气荚膜梭菌的胆固醇结合θ毒素和链霉蛋白酶也能抑制EA玫瑰花结的形成，但作用不如鞘磷脂酶强。脂质特异性试剂也能抑制白细胞向酪蛋白和变性人血清白蛋白的趋化迁移，而蛋白酶和糖苷酶则不能。这些结果表明膜脂是Fc片段结合位点及某些趋化因子的重要组成成分，并指出鞘磷脂在这种结合中起重要作用。
