泰国人类和肉鸡中肠炎沙门氏菌分离株的噬菌体分型和脉冲场凝胶电泳流行病学分析
Epidemiological analysis of Salmonella enteritidis isolates from humans and broiler chickens in Thailand by phage typing and pulsed-field gel electrophoresis.
作者信息
Boonmar S, Bangtrakulnonth A, Pornrunangwong S, Terajima J, Watanabe H, Kaneko K, Ogawa M
机构信息
Faculty of Veterinary Medicine, Kasetsart University, Bangkhen, Bangcock, Thailand.
出版信息
J Clin Microbiol. 1998 Apr;36(4):971-4. doi: 10.1128/JCM.36.4.971-974.1998.
Abstract
To determine the phage types (PT) of Salmonella enteritidis found in Thailand and to clarify the potential for human infection by S. enteritidis in broiler chicken meat, human and poultry isolates taken from Thailand between 1990 and 1997 were phage typed and analyzed by pulsed-field gel electrophoresis (PFGE). Ten different PT were found among the 302 isolates phage typed, with PT 4 being the most frequent in human (73.9%) and poultry (76.2%) isolates, followed by PT 1 (8.0%), 8 (3.6%), and 7a (2.2%) in human isolates and by PT 7a (4.9%), 1 (3.7%), and 12 (2.4%) in poultry isolates. Of the 53 isolates analyzed by PFGE, 45 showed an indistinguishable pattern (pattern A) by BlnI-digested PFGE and the other 8 isolates showed a very similar pattern that differed by only a few bands. These results indicate the spread of a genetically identical clone of S. enteritidis in humans and poultry in Thailand.
摘要
为确定泰国肠炎沙门氏菌的噬菌体类型（PT），并阐明肉鸡鸡肉中肠炎沙门氏菌对人类的潜在感染风险，对1990年至1997年期间取自泰国的人类和家禽分离株进行噬菌体分型，并通过脉冲场凝胶电泳（PFGE）进行分析。在302株经噬菌体分型的分离株中发现了10种不同的PT，其中PT4在人类（73.9%）和家禽（76.2%）分离株中最为常见，其次是人类分离株中的PT1（8.0%）、PT8（3.6%）和PT7a（2.2%），以及家禽分离株中的PT7a（4.9%）、PT1（3.7%）和PT12（2.4%）。在经PFGE分析的53株分离株中，45株在BlnI酶切PFGE中显示出无法区分的图谱（图谱A），另外8株显示出非常相似的图谱，仅在几条条带上有所不同。这些结果表明，泰国人类和家禽中存在基因相同的肠炎沙门氏菌克隆株的传播。
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