Pastey M K, Samal S K
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742, USA.
J Clin Microbiol. 1998 Apr;36(4):1105-8. doi: 10.1128/JCM.36.4.1105-1108.1998.
The fusion (F) protein of bovine respiratory syncytial virus (BRSV) was expressed by using a baculovirus vector. Antigenicity was tested by immunofluorescence analysis with F-specific monoclonal and polyclonal antibodies. Antibodies to recombinant F protein raised in a rabbit neutralized BRSV and human respiratory syncytial virus infectivity when tested in a plaque reduction assay. The recombinant F protein was evaluated as a source of antigen in an enzyme-linked immunosorbent assay (ELISA), and this ELISA was compared with the virus neutralization (VN) test for detecting BRSV antibodies in 10 consecutive serum samples from four calves vaccinated with a live modified BRSV vaccine and from two nonvaccinated control calves. The ELISA with the baculovirus-expressed F protein as an antigen compared favorably with the VN test and is a rapid, sensitive, and specific method for detecting serum antibodies to BRSV.
利用杆状病毒载体表达牛呼吸道合胞病毒（BRSV）的融合（F）蛋白。通过用F特异性单克隆抗体和多克隆抗体进行免疫荧光分析来检测抗原性。在蚀斑减少试验中检测时，兔体内产生的针对重组F蛋白的抗体中和了BRSV和人呼吸道合胞病毒的感染性。在酶联免疫吸附测定（ELISA）中评估重组F蛋白作为抗原的来源，并将该ELISA与病毒中和（VN）试验进行比较，以检测来自4头接种了减毒活BRSV疫苗的小牛和2头未接种对照小牛的10份连续血清样本中的BRSV抗体。以杆状病毒表达的F蛋白作为抗原的ELISA与VN试验相比具有优势，是一种检测BRSV血清抗体的快速、灵敏且特异的方法。
