Marin-Garcia J, Ananthakrishnan R, Goldenthal M J
The Molecular Cardiology Institute, Highland Park, NJ 08904, USA.
Mol Cell Biochem. 1998 Feb;179(1-2):21-6. doi: 10.1023/a:1006839831141.
Little information is presently available concerning mitochondrial respiratory and oxidative phosphorylation function in the normal human heart during growth and development. We investigated the levels of specific mitochondrial enzyme activities and content during cardiac growth and development from the early neonatal period (10-20 days) to adulthood (67 years). Biochemical analysis of enzyme specific activities and content and mitochondrial DNA (mtDNA) copy number was performed with left ventricular tissues derived from 30 control individuals. The levels of cytochrome c oxidase (COX) and complex V specific activity, mtDNA copy number and COX subunit II content remained unchanged in contrast to increased citrate synthase (CS) activity and content. The developmental increase in CS activity paralleled increasing CS polypeptide content, but was neither related to overall increases in mitochondrial number nor coordinately regulated with mitochondrial respiratory enzyme activities. Our findings of unchanged levels of cardiac mitochondrial respiratory enzyme activity during the progression from early childhood to older adult contrasts with the age-specific regulation found with CS, a Krebs cycle mitochondrial enzyme.
目前关于正常人类心脏在生长发育过程中线粒体呼吸和氧化磷酸化功能的信息很少。我们研究了从新生儿早期（10 - 20天）到成年期（67岁）心脏生长发育过程中特定线粒体酶活性和含量的水平。对来自30名对照个体的左心室组织进行了酶比活性、含量以及线粒体DNA（mtDNA）拷贝数的生化分析。与柠檬酸合酶（CS）活性和含量增加形成对比的是，细胞色素c氧化酶（COX）和复合物V比活性、mtDNA拷贝数以及COX亚基II含量保持不变。CS活性的发育性增加与CS多肽含量的增加平行，但既与线粒体数量的总体增加无关，也与线粒体呼吸酶活性没有协同调节关系。我们发现，从幼儿期到老年期，心脏线粒体呼吸酶活性水平没有变化，这与柠檬酸合酶（一种三羧酸循环线粒体酶）的年龄特异性调节情况形成了对比。
