Castelnuovo J, Tjan A H, Liu P
Advanced Education Program in Prosthodontics, Loma Linda University, School of Dentistry, California, USA.
Am J Dent. 1996 Dec;9(6):245-8.
To measure and compare the microleakage of Class V resin composite restorations using the following three pairs of multi- and simplified-step dentin bonding systems (DBS): OptiBond vs. OptiBond FL, All-Bond 2 vs. One-Step, and Tenure vs. Tenure Quik.
V-shaped cavities were prepared in the mesial and distal surfaces of 30 extracted human mandibular molars with margins in both enamel and cementum, and randomly assigned into three equal groups of 10 each. All DBSs were applied strictly following manufacturers' specifications before three incremental layers of resin composite (Pertac-Hybrid) were applied in all cavities. Specimens were stored for 7 days in 37 degrees C water, thermocycled for 300 cycles between 5 degrees C and 55 degrees C, immersed in 0.5% basic fuchsin solution for 24 hours, and embedded in resin before being sectioned longitudinally in multiple sections. Dye solution penetration at the tooth/composite interface was scored from 0 to 4 under a microscope at x100 at both enamel (E) and cementum (C) margins. Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests with alpha = 0.05.
OptiBond FL and One-Step showed less microleakage at the cementum margins compared to their multi-step versions. At the enamel margins Tenure Quik showed less microleakage compared to Tenure, and none of the other DBSs tested showed significant dye penetration at that interface. All DBSs showed significantly less microleakage at the enamel margins compared to the cementum margins except for OptiBond FL and One-Step that did not show any significant dye penetration at the cementum margins as well.
使用以下三对多步骤和简化步骤牙本质粘结系统（DBS）测量并比较V类树脂复合体修复体的微渗漏情况：OptiBond与OptiBond FL、全粘结2型与一步法、Tenure与Tenure Quik。
在30颗拔除的人类下颌磨牙的近中面和远中面制备V形洞，边缘位于釉质和牙骨质，随机分为三组，每组10颗。在所有窝洞应用三层递增的树脂复合体（Pertac-Hybrid）之前，严格按照制造商的规格应用所有DBS。标本在37℃水中储存7天，在5℃至55℃之间进行300次热循环，浸入0.5%碱性品红溶液中24小时，然后嵌入树脂中，再纵向切成多个切片。在显微镜下以100倍放大倍数在釉质（E）和牙骨质（C）边缘对牙齿/复合体界面处的染料溶液渗透情况从0到4进行评分。数据采用Kruskal-Wallis检验和Mann-Whitney U检验进行分析，α = 0.05。
与它们的多步骤版本相比，OptiBond FL和一步法在牙骨质边缘显示出较少的微渗漏。在釉质边缘，Tenure Quik比Tenure显示出更少的微渗漏，并且测试的其他DBS在该界面均未显示出明显的染料渗透。除了OptiBond FL和一步法在牙骨质边缘也未显示出任何明显的染料渗透外，所有DBS在釉质边缘的微渗漏均显著少于牙骨质边缘。
