来自乳酸乳球菌乳酸亚种ML3的接合元件pRS01上的转移起始位点（oriT）。
An origin of transfer (oriT) on the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3.
作者信息
Mills D A, Phister T G, Dunny G M, McKay L L
机构信息
Department of Microbiology, University of Minnesota, Minneapolis 55455-0312, USA.
出版信息
Appl Environ Microbiol. 1998 Apr;64(4):1541-4. doi: 10.1128/AEM.64.4.1541-1544.1998.
Abstract
Previous analysis of the Tra1 region of the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3 suggested that an origin of transfer (oriT) was present. Deletion derivatives of this cloned Tra1 region were assayed for mobilization in the presence of the wild-type pRS01 element in trans. The pRS01 oriT was localized to a 446-nucleotide segment in the intergenic region between open reading frames ltrD and ltrE. Sequence analysis of this region revealed a cluster of direct and inverted repeat structures characteristic of oriT regions associated with other conjugative systems.
摘要
先前对乳酸乳球菌乳亚种ML3的接合元件pRS01的Tra1区域进行的分析表明，存在转移起始点（oriT）。在反式存在野生型pRS01元件的情况下，对该克隆的Tra1区域的缺失衍生物进行了转移检测。pRS01 oriT定位于开放阅读框ltrD和ltrE之间基因间区域的一个446核苷酸片段。对该区域的序列分析揭示了与其他接合系统相关的oriT区域特有的直接和反向重复结构簇。
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