染料木黄酮和染料木苷对老年大鼠股骨干骺端组织骨代谢的合成代谢作用：锌可增强染料木黄酮的作用。

Anabolic effect of genistein and genistin on bone metabolism in the femoral-metaphyseal tissues of elderly rats: the genistein effect is enhanced by zinc.

作者信息

Yamaguchi M, Gao Y H

机构信息

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka City, Japan.

出版信息

Mol Cell Biochem. 1998 Jan;178(1-2):377-82. doi: 10.1023/a:1006809031836.

DOI:10.1023/a:1006809031836

PMID:9546622

Abstract

The effect of genistein and genistin on bone components in the femoral-metaphyseal tissues obtained from elderly female rats was investigated in vitro. The metaphyseal tissues were cultured for 24 h in a medium containing either vehicle, genistein (10(-8)-10(-5) M) or genistin (10(-7)-10(-5) M). The presence of genistein or genistin caused a significant increase in alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in the metaphyseal tissues. The effect of genistein was greater than that of genistin. The bone components increased by genistein (10(-5) M) or genistin (10(-5) M) were completely blocked by the presence of cycloheximide (10(-6) M). The presence of zinc sulfate (10(-5) M) caused a significant increase in the genistein (10(-5) M)-elevated alkaline phosphatase activity, DNA and calcium contents. The enhancement with zinc was not seen by genistin (10(-5) M). The stimulatory effect of zinc on the genistein-induced increase in bone components of the metaphyseal tissues was completely blocked by the presence of cycloheximide (10(-6) M). The present results suggest that genistein and genistin have an anabolic effect on bone metabolism in the femoral-metaphyseal tissues of elderly rats, and that the genistein effect is enhanced by zinc, an essential trace element.

摘要

体外研究了染料木黄酮和染料木苷对老年雌性大鼠股骨-干骺端组织中骨成分的影响。将干骺端组织在含有载体、染料木黄酮（10⁻⁸ - 10⁻⁵ M）或染料木苷（10⁻⁷ - 10⁻⁵ M）的培养基中培养24小时。染料木黄酮或染料木苷的存在导致干骺端组织中碱性磷酸酶活性、脱氧核糖核酸（DNA）和钙含量显著增加。染料木黄酮的作用大于染料木苷。染料木黄酮（10⁻⁵ M）或染料木苷（10⁻⁵ M）增加的骨成分被放线菌酮（10⁻⁶ M）完全阻断。硫酸锌（10⁻⁵ M）的存在导致染料木黄酮（10⁻⁵ M）升高的碱性磷酸酶活性、DNA和钙含量显著增加。染料木苷（10⁻⁵ M）未观察到锌的增强作用。锌对染料木黄酮诱导的干骺端组织骨成分增加的刺激作用被放线菌酮（10⁻⁶ M）完全阻断。目前的结果表明，染料木黄酮和染料木苷对老年大鼠股骨-干骺端组织的骨代谢具有合成代谢作用，并且染料木黄酮的作用被必需微量元素锌增强。