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源自体外磷酸化大肠杆菌RNA聚合酶的磷酸肽与DNA结合并影响DNA转录。

Phosphopeptides derived from in vitro phosphorylated E. coli RNA polymerase bind to DNA and affect DNA transcription.

作者信息

Cardellini E, Piccinini G, Gianfranceschi G L

机构信息

Dipartimento di Biologia Cellulare e Molecolare, Sezione Fisiologia e Biofisica, Università di Perugia, Italy.

出版信息

Mol Cell Biochem. 1998 Jan;178(1-2):393-6. doi: 10.1023/a:1006811413681.

DOI:10.1023/a:1006811413681
PMID:9546625
Abstract

E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.

摘要

用蛋白激酶CKII使大肠杆菌RNA聚合酶磷酸化，然后使其与pBR322结合。在用蛋白酶K消化RNA聚合酶-pBR322复合物后，提取并分析仍与DNA结合的磷酸肽。这些磷酸肽能够再次与DNA结合并抑制转录。

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引用本文的文献

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本文引用的文献

1
In vitro phosphorylation of proteins tightly bound to DNA by protein kinase NII.蛋白激酶NII对紧密结合于DNA的蛋白质进行体外磷酸化作用。
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2
Phosphorylation of deoxyribonucleic acid dependent RNA polymerase II by nuclear protein kinase NII: mechanism of enhanced ribonucleic acid synthesis.核蛋白激酶NII对脱氧核糖核酸依赖性核糖核酸聚合酶II的磷酸化作用：核糖核酸合成增强的机制
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Topoisomerase I phosphorylation in vitro and in rapidly growing Novikoff hepatoma cells.
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Synthetic model peptides phosphorylated in vitro by NII kinase and cation-mediated interactions with DNA.通过NII激酶在体外磷酸化并与DNA进行阳离子介导相互作用的合成模型肽。
Physiol Chem Phys Med NMR. 1989;21(4):289-94.
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Casein kinase 2: an 'eminence grise' in cellular regulation?
Biochim Biophys Acta. 1990 Sep 24;1054(3):267-84. doi: 10.1016/0167-4889(90)90098-x.
6
Chemical and enzymatic analysis of covalent bonds between peptides and chromosomal DNA.肽与染色体DNA之间共价键的化学和酶学分析
Nucleic Acids Res. 1991 Dec 11;19(23):6391-8. doi: 10.1093/nar/19.23.6391.
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Protein kinase NII from calf thymus chromatin. Isolation, characterization and some functional properties.
Int J Biochem. 1992 Nov;24(11):1785-92. doi: 10.1016/0020-711x(92)90129-o.
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Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro.小牛胸腺中存在一种在体外控制DNA转录的肽因子的证据。
Biochim Biophys Acta. 1975 Nov 18;414(1):9-19. doi: 10.1016/0005-2787(75)90121-5.