源自体外磷酸化大肠杆菌RNA聚合酶的磷酸肽与DNA结合并影响DNA转录。
Phosphopeptides derived from in vitro phosphorylated E. coli RNA polymerase bind to DNA and affect DNA transcription.
作者信息
Cardellini E, Piccinini G, Gianfranceschi G L
机构信息
Dipartimento di Biologia Cellulare e Molecolare, Sezione Fisiologia e Biofisica, Università di Perugia, Italy.
出版信息
Mol Cell Biochem. 1998 Jan;178(1-2):393-6. doi: 10.1023/a:1006811413681.
PMID:9546625
Abstract
E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR322 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.
摘要
用蛋白激酶CKII使大肠杆菌RNA聚合酶磷酸化，然后使其与pBR322结合。在用蛋白酶K消化RNA聚合酶-pBR322复合物后，提取并分析仍与DNA结合的磷酸肽。这些磷酸肽能够再次与DNA结合并抑制转录。
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