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小牛胸腺中存在一种在体外控制DNA转录的肽因子的证据。

Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro.

作者信息

Gianfranceschi G L, Amici D, Guglielmi L

出版信息

Biochim Biophys Acta. 1975 Nov 18;414(1):9-19. doi: 10.1016/0005-2787(75)90121-5.

DOI:10.1016/0005-2787(75)90121-5
PMID:1191703
Abstract

A thymic factor causes a strong inhibition of the DNA-directed RNA polymerase reaction in vitro. The active factor was isolated from aqueous ultrafiltered thymus extracts and purified by means of chromatography on DEAE-cellulose and then on Dowex 50 WX2. The purified thymic factor was characterized as a peptide of low molecular weight (less than 5000). The biological activity of the thymic factor cannot be attributed to the presence of a nuclease or of a histone fragment. The RNA synthesis is controlled by this factor by means of electrostatic interactions between the peptide compound and DNA. Inhibitory activity on RNA synthesis was absent from kidney extracts.

摘要

一种胸腺因子在体外能强烈抑制DNA指导的RNA聚合酶反应。活性因子是从胸腺水超滤提取物中分离出来的，并通过DEAE -纤维素柱层析，然后再通过Dowex 50 WX2柱层析进行纯化。纯化后的胸腺因子被鉴定为低分子量（小于5000）的肽。胸腺因子的生物活性不能归因于核酸酶或组蛋白片段的存在。该因子通过肽化合物与DNA之间的静电相互作用来控制RNA合成。肾提取物不存在对RNA合成的抑制活性。

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Evidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro.小牛胸腺中存在一种在体外控制DNA转录的肽因子的证据。
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3
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