Rolf C, Behre H M, Cooper T G, Koppers B, Nieschlag E
Institute of Reproductive Medicine of the University, Münster, Germany.
Fertil Steril. 1998 Apr;69(4):727-34. doi: 10.1016/s0015-0282(97)00570-0.
To examine the predictive value of creatine kinase in human spermatozoa and seminal plasma from patients treated in an IVF program.
Prospective, blind clinical study.
Male infertility clinic.
PATIENT(S): Seventy-three patients and 32 fathers (age, <45 years).
INTERVENTION(S): Determination of creatine kinase activity in seminal plasma, washed spermatozoa, and swim-up purified spermatozoa from patients treated in an IVF program.
MAIN OUTCOME MEASURE(S): Creatine kinase activity in seminal plasma, washed spermatozoa, and swim-up purified spermatozoa.
RESULT(S): Creatine kinase activity in washed spermatozoa correlated significantly with normal sperm morphology. No significant correlations were found between creatine kinase activity or creatine kinase isoenzyme ratio in seminal plasma, washed spermatozoa, or swim-up purified spermatozoa and success in the IVF program. The percentage of spermatozoa with normal morphology was significantly lower in patients whose sperm did not fertilize than in patients whose sperm did fertilize oocytes and in the control group of proven fathers.
CONCLUSION(S): Total creatine kinase activity and creatine kinase isoenzyme distribution are not sperm function markers for prediction of male fertility in IVF treatment.
研究肌酸激酶在接受体外受精（IVF）治疗患者的人类精子及精浆中的预测价值。
前瞻性、盲法临床研究。
男性不育诊所。
73例患者及32名父亲（年龄<45岁）。
测定接受IVF治疗患者的精浆、洗涤精子及上游法纯化精子中的肌酸激酶活性。
精浆、洗涤精子及上游法纯化精子中的肌酸激酶活性。
洗涤精子中的肌酸激酶活性与正常精子形态显著相关。精浆、洗涤精子或上游法纯化精子中的肌酸激酶活性或肌酸激酶同工酶比例与IVF治疗成功率之间未发现显著相关性。精子未受精患者的正常形态精子百分比显著低于精子使卵母细胞受精的患者及已证实有生育能力的父亲对照组。
总肌酸激酶活性及肌酸激酶同工酶分布并非IVF治疗中预测男性生育能力的精子功能标志物。
