大鼠脑局部区域中3,4-二羟基苯乙酸和高香草酸的含量：荧光法同步测定及药物的影响

Regional rat brain levels of 3,4-dihydroxyphenylacetic acid and homovanillic acid: concurrent fluorometric measurement and influence of drugs.

作者信息

Westerink B H, Korf J

出版信息

Eur J Pharmacol. 1976 Aug;38(2):281-91. doi: 10.1016/0014-2999(76)90331-9.

Abstract

A concurrent semi-automatic fluorometric assay technique for 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), is described. The method is based on a rapid manually performed isolation of DOPAC and HVA on small columns of Sephadex G-10 followed by an automated fluorometric detection with a continuous flow system. DOPAC and HVA were measured in the corpus striatum, nucleus accumbens and olfactory tubercle of the rat, under normal conditions and after treatment with amphetamine, apomorphine, clozapine, haloperidol, morphine, oxotremorine, pargyline, probenecid, sulpiride and thioridazine. Clozapine, morphine, sulpiride and oxotremorine induced the most pronounced rise of DA metabolites in the nucleus accumbens. Probenecid produced a DOPAC accumulation in the nucleus accumbens. Striking differences were observed between the DOPAC/HVA ratios in the different structures of control animals. The concurrent assay enables a rapid screening of the action of drugs in regional DA metabolism.

摘要

本文描述了一种用于同时测定3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）的半自动荧光测定技术。该方法基于在Sephadex G-10小柱上快速手动分离DOPAC和HVA，然后采用连续流动系统进行自动荧光检测。在正常条件下以及用苯丙胺、阿扑吗啡、氯氮平、氟哌啶醇、吗啡、氧化震颤素、帕吉林、丙磺舒、舒必利和硫利达嗪处理后，测定大鼠纹状体、伏隔核和嗅结节中的DOPAC和HVA。氯氮平、吗啡、舒必利和氧化震颤素使伏隔核中多巴胺代谢产物的升高最为明显。丙磺舒导致伏隔核中DOPAC蓄积。在对照动物的不同结构中，DOPAC/HVA比值存在显著差异。同时测定能够快速筛选药物对局部多巴胺代谢的作用。