Warwick R O, Schnell R C
Eur J Pharmacol. 1976 Aug;38(2):329-35. doi: 10.1016/0014-2999(76)90336-8.
Experiments were conducted to determine if the acute hypothermia elicited by morphine in the rat resulted from morphine-induced inhibition of serotonin reuptake into hypothalamic nerve-endings. The acute hypothermia elicited by morphine (30 mg/kg, s.c.) was reversed by pretreatment with naloxone (10 mg/kg, s.c.), a narcotic antagonist, which alone did not alter body temperature. In vitro uptake of serotonin into synaptosomes prepared from rat hypothalamus was inhibited 53% by the addition of morphine (7 X 10(-4) M) and 53.9% by naloxone (1 X 10(-3) M). Simultaneous addition of both drugs in these same concentrations further inhibited synaptosomal serotonin uptake 77.8%. Uptake of serotonin uptake into synaptosomes isolated from rats injected with morphine (30 mg/kg, s.c.) was not altered as compared to controls. These data suggest that the acute hypothermic acition in morphine in the rat is not related to an inhibition of the serotonin reuptake mechanism in hypothalamic nerve-endings.
进行实验以确定吗啡在大鼠中引发的急性体温过低是否源于吗啡诱导的血清素摄入下丘脑神经末梢的抑制作用。用麻醉拮抗剂纳洛酮（10mg/kg，皮下注射）预处理可逆转吗啡（30mg/kg，皮下注射）引发的急性体温过低，单独使用纳洛酮不会改变体温。添加吗啡（7×10⁻⁴M）可使从大鼠下丘脑制备的突触体中血清素的体外摄取抑制53%，添加纳洛酮（1×10⁻³M）可抑制53.9%。同时添加相同浓度的两种药物可进一步抑制突触体血清素摄取77.8%。与对照组相比，从注射吗啡（30mg/kg，皮下注射）的大鼠中分离出的突触体中血清素的摄取没有改变。这些数据表明，吗啡在大鼠中的急性体温过低作用与下丘脑神经末梢中血清素摄取机制的抑制无关。
