Vunjak-Novakovic G, Obradovic B, Martin I, Bursac P M, Langer R, Freed L E
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Biotechnol Prog. 1998 Mar-Apr;14(2):193-202. doi: 10.1021/bp970120j.
Cell seeding of three-dimensional polymer scaffolds is the first step of the cultivation of engineered tissues in bioreactors. Seeding requirements of large scaffolds to make implants for potential clinical use include: (a) high yield, to maximize the utilization of donor cells, (b) high kinetic rate, to minimize the time in suspension for anchorage-dependent and shear-sensitive cells, and (c) high and spatially uniform distribution of attached cells, for rapid and uniform tissue regeneration. Highly porous, fibrous polyglycolic acid scaffolds, 5-10 mm in diameter and 2-5 mm thick, were seeded with bovine articular chondrocytes in well-mixed spinner flasks. Essentially, all cells attached throughout the scaffold volume within 1 day. Mixing promoted the formation of 20-32-micron diameter cell aggregates that enhanced the kinetics of cell attachment without compromising the uniformity of cell distribution. The kinetics and possible mechanisms of cell seeding were related to the formation of cell aggregates by a simple mathematical model that can be used to optimize seeding conditions for cartilage tissue engineering.
将细胞接种到三维聚合物支架上是在生物反应器中培养工程组织的第一步。为制造潜在临床应用的植入物而对大型支架进行接种的要求包括：（a）高产量，以最大限度地利用供体细胞；（b）高动力学速率，以尽量减少贴壁依赖性和剪切敏感性细胞在悬浮液中的停留时间；（c）附着细胞的高且空间均匀分布，以实现快速且均匀的组织再生。将直径为5 - 10毫米、厚度为2 - 5毫米的高度多孔纤维聚乙醇酸支架在充分混合的旋转瓶中接种牛关节软骨细胞。基本上，所有细胞在1天内附着于整个支架体积内。混合促进了直径为20 - 32微米的细胞聚集体的形成，这增强了细胞附着的动力学，同时不影响细胞分布的均匀性。细胞接种的动力学和可能机制通过一个简单的数学模型与细胞聚集体的形成相关，该模型可用于优化软骨组织工程的接种条件。
