Nicolet J, Miserez R, Gonçalves R, Regalla J, Griot C, Bensaide A, Krampe M, Frey J
Institute for Veterinary Bacteriology, University of Bern, Switzerland.
Vet Microbiol. 1998 Jan 16;59(2-3):109-22. doi: 10.1016/s0378-1135(97)00184-3.
The course of immune reactions of the manifold antigens of Mycoplasma mycoides subsp. mycoides small colony type (SC) was analysed in serum and bronchial lavage of cattle experimentally infected with the African strain Afadé and the European strain L2 using Western-blots and complement fixation. Western-blot analysis of total antigens of both strains with sera from animals infected with the homologous and heterologous strain revealed the common dominant immunogenic antigens with the molecular masses of 110, 95, 85, 80, 72, 62, 48 and 39 kDa. The sequential sampling of the blood and bronchial lavages before and after contact infections allowed us to identify the antigens of 85, 80, 72, 48 and 39 kDa as particularly early immunogens. The IgA Western blots of the bronchial lavages showed distinct, early and persistent reactions to the 110, 85, 80, 72, 48 and 45 kDa proteins. These proteins were the predominant lipoproteins as determined by [14C]palmitic acid labelling. Only relatively weak reactions of the bronchial lavages were detected with IgG. In general immune responses were significantly stronger in the animals infected with the African strain Afadé, which gave positive results two weeks after contact infection. In contrast, the animals infected with the European strain L2 induced much lower reactions with a delay of three months after contact infection. In one animal strain L2 caused no sero-conversion and no infection. The results indicate a difference in virulence between the African strain Afadé and the European strain L2.
利用蛋白质免疫印迹法和补体结合试验，对实验感染非洲菌株阿法德（Afadé）和欧洲菌株L2的牛血清及支气管灌洗液中，蕈状支原体丝状亚种小菌落型（SC）多种抗原的免疫反应过程进行了分析。用同源和异源菌株感染动物的血清对两种菌株的总抗原进行蛋白质免疫印迹分析，结果显示，分子量为110、95、85、80、72、62、48和39 kDa的抗原是共同的主要免疫原性抗原。在接触感染前后对血液和支气管灌洗液进行连续采样，使我们能够确定分子量为85、80、72、48和39 kDa的抗原为特别早期的免疫原。支气管灌洗液的IgA蛋白质免疫印迹显示，对分子量为110、85、80、72、48和45 kDa的蛋白质有明显、早期且持续的反应。通过[14C]棕榈酸标记确定，这些蛋白质是主要的脂蛋白。支气管灌洗液中仅检测到相对较弱的IgG反应。总体而言，感染非洲菌株阿法德的动物免疫反应明显更强，接触感染两周后呈阳性结果。相比之下，感染欧洲菌株L2的动物反应则低得多，接触感染三个月后才出现反应。在一只动物中，菌株L2未引起血清转化，也未导致感染。结果表明非洲菌株阿法德和欧洲菌株L2在毒力上存在差异。
