Roark Christina L, Aydintug M Kemal, Lewis Julie, Yin Xiang, Lahn Michael, Hahn Youn-Soo, Born Willi K, Tigelaar Robert E, O'Brien Rebecca L
Integrated Department of Immunology, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA.
J Leukoc Biol. 2004 Jan;75(1):68-75. doi: 10.1189/jlb.0703326. Epub 2003 Oct 2.
The V gamma 6/V delta 1(+) cells, the second murine gamma delta T cell subset to arise in the thymus, express a nearly invariant T cell receptor (TCR), colonize select tissues, and expand preferentially in other tissues during inflammation. These cells are thought to help in regulating the inflammatory response. Until now, V gamma 6/V delta 1(+) cells have only been detectable indirectly, by expression of V gamma 6-encoding mRNA. Here, we report that 17D1, a monoclonal antibody, which detects the related epidermis-associated V gamma 5/V delta 1(+) TCR, will also bind the V gamma 6/V delta 1(+) cells if their TCR is first complexed to an anti-C delta antibody. Features of this special condition for recognition suggest the possibility that an alternate structure exists for the V gamma 6/V delta 1 TCR, which is stabilized upon binding to the anti-C delta antibody. Using the 17D1 antibody as means to track this gamma delta T cell subset by flow cytometry, we discovered that the response of V gamma 6/V delta 1(+) cells during inflammation often far exceeds that of other subsets and that the responding V gamma 6/V delta 1(+) cells display a strikingly uniform activation/memory phenotype compared with other gamma delta T cell subsets.
Vγ6/Vδ1(+)细胞是在胸腺中出现的第二个小鼠γδT细胞亚群，表达几乎恒定的T细胞受体（TCR），定位于特定组织，并在炎症期间在其他组织中优先扩增。这些细胞被认为有助于调节炎症反应。到目前为止，Vγ6/Vδ1(+)细胞只能通过编码Vγ6的mRNA的表达间接检测到。在此，我们报告，单克隆抗体17D1可检测相关的表皮相关Vγ5/Vδ1(+)TCR，如果Vγ6/Vδ1(+)细胞的TCR首先与抗Cδ抗体形成复合物，该抗体也会与Vγ6/Vδ1(+)细胞结合。这种特殊识别条件的特征表明，Vγ6/Vδ1 TCR可能存在另一种结构，该结构在与抗Cδ抗体结合后会稳定下来。使用17D1抗体通过流式细胞术追踪这个γδT细胞亚群，我们发现炎症期间Vγ6/Vδ1(+)细胞的反应通常远远超过其他亚群，并且与其他γδT细胞亚群相比，反应性Vγ6/Vδ1(+)细胞表现出惊人的一致激活/记忆表型。
