人成纤维细胞中DNA双链断裂修复的原位可视化

In situ visualization of DNA double-strand break repair in human fibroblasts.

作者信息

Nelms B E, Maser R S, MacKay J F, Lagally M G, Petrini J H

机构信息

Laboratory of Genetics and Department of Medical Physics, University of Wisconsin Medical School, Madison, WI 53706, USA.

出版信息

Science. 1998 Apr 24;280(5363):590-2. doi: 10.1126/science.280.5363.590.

DOI:10.1126/science.280.5363.590

PMID:9554850

Abstract

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

摘要

开发了一种方法来检测完整细胞内的DNA修复。使用超软X射线在人成纤维细胞的特定亚核区域诱导DNA双链断裂（DSB），并在这些位点观察DNA修复情况。在DNA修复的初始阶段，DSB保持在固定位置，DSB修复蛋白hMre11在30分钟内迁移到损伤位点。相比之下，人RecA同源物hRad51并未定位于DNA损伤位点，这一发现与这些蛋白质在DNA修复中的不同作用一致。