Ashkenazi-Ezra R, Henderson N
In Vitro. 1976 Jun;12(6):436-41.
Conditioned media of 21 mammalian cell lines have been examined by electron microscopic and biochemical techniques for the presence of a minute ring-shaped nucleoprotein particle (RSP). Electron microscopy showed the presence of RSP in 20 of the 21 cell lines tested. Comparative analyses of sex cell cultures for radioactive RSP, following 3H-thymidine incorporation, suggested that quantitative differences exist in the amount of RSP detectable in conditioned media of normal and pathologic cells. The cell lines tested included cells of different morphologies, origin and function
已通过电子显微镜和生化技术检查了21种哺乳动物细胞系的条件培养基，以确定是否存在微小的环形核蛋白颗粒（RSP）。电子显微镜显示，在测试的21种细胞系中有20种存在RSP。在掺入3H-胸腺嘧啶核苷后，对生殖细胞培养物中的放射性RSP进行比较分析，结果表明，正常细胞和病理细胞的条件培养基中可检测到的RSP量存在定量差异。所测试的细胞系包括具有不同形态、来源和功能的细胞。
