Duan X, Nauwynck H J, Favoreel H W, Pensaert M B
Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
J Virol. 1998 May;72(5):4520-3. doi: 10.1128/JVI.72.5.4520-4523.1998.
To identify the receptor which may determine the macrophage tropism of porcine reproductive and respiratory syndrome virus (PRRSV), monoclonal antibodies (MAbs) against porcine alveolar macrophages (PAM) were produced. Two MAbs (41D3 and 41D5) which completely blocked PRRSV infection of PAM were further characterized. It was found that they reduce the attachment of PRRSV to PAM and immunoprecipitate a 210-kDa membrane protein from PAM. This protein was detected on the cell membranes of PAM but not of PRRSV-nonpermissive cells. A colocalization was found between the reactive sites of MAb 41D3 and PRRSV on PAM membranes. All PRRSV-infected cells in tissues of experimentally infected pigs reacted with MAb 41D3. Taken together, all these data suggest that the identified 210-kDa membrane protein is a putative receptor for PRRSV on porcine macrophages.
为了鉴定可能决定猪繁殖与呼吸综合征病毒（PRRSV）巨噬细胞嗜性的受体，制备了针对猪肺泡巨噬细胞（PAM）的单克隆抗体（MAb）。进一步鉴定了两种能完全阻断PRRSV感染PAM的单克隆抗体（41D3和41D5）。结果发现，它们减少了PRRSV与PAM的附着，并从PAM中免疫沉淀出一种210 kDa的膜蛋白。这种蛋白在PAM的细胞膜上被检测到，但在PRRSV非允许细胞的细胞膜上未被检测到。在PAM膜上发现单克隆抗体41D3的反应位点与PRRSV之间存在共定位。实验感染猪组织中的所有PRRSV感染细胞均与单克隆抗体41D3发生反应。综上所述，所有这些数据表明，鉴定出的210 kDa膜蛋白是PRRSV在猪巨噬细胞上的一种假定受体。
