Wensvoort G, Terpstra C, Pol J M, ter Laak E A, Bloemraad M, de Kluyver E P, Kragten C, van Buiten L, den Besten A, Wagenaar F
Central Veterinary Institute, Virology Department, Lelystad, The Netherlands.
Vet Q. 1991 Jul;13(3):121-30. doi: 10.1080/01652176.1991.9694296.
In early 1991, the Dutch pig-industry was struck by the so-called mystery swine disease. Large-scale laboratory investigations were undertaken to search for the etiological agent. We focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. The mycoplasmas M. hyosynoviae, M. hyopneumoniae, and Acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 were isolated from individual pigs. An unknown agent, however, was isolated from 16 of 20 piglets and from 41 of 63 sows. This agent was characterised as a virus and designated Lelystad virus. No relationship between this virus and other viruses has yet been established. Of 165 sows reportedly afflicted by the disease, 123 (75 per cent) seroconverted to Lelystad virus, whereas less than 10 per cent seroconverted to any of the other virus isolates or to the known viral pathogens. Antibodies directed against Lelystad virus were also found in pigs with mystery swine disease in England, Germany, and in the United States. We conclude that infection with Lelystad virus is the likely cause of mystery swine disease.
1991年初，荷兰养猪业遭受了所谓的神秘猪病侵袭。开展了大规模实验室调查以寻找病原体。我们着重于分离病毒和支原体，并检测患病母猪的配对血清中针对十种已知猪病毒的抗体。从个别猪身上分离出了猪滑膜支原体、猪肺炎支原体和莱氏无胆甾原体，以及脑心肌炎病毒和2型及7型猪肠道病毒。然而，从20头仔猪中的16头以及63头母猪中的41头身上分离出了一种未知病原体。该病原体被鉴定为一种病毒，并命名为莱利斯塔德病毒。尚未确定这种病毒与其他病毒之间的关系。据报道，在165头患该病的母猪中，123头（75%）血清转化为莱利斯塔德病毒，而血清转化为其他任何病毒分离株或已知病毒病原体的比例不到10%。在英国、德国和美国患有神秘猪病的猪身上也发现了针对莱利斯塔德病毒的抗体。我们得出结论，感染莱利斯塔德病毒可能是神秘猪病的病因。
