Zhao X L, Wu J H
Department of Environmental Toxicology, Chinese Academy of Preventive Medicine, Beijing, China.
Biomed Environ Sci. 1998 Mar;11(1):1-6.
This study was carried out to observe the effects of sodium fluoride on acetylcholinesterase (AChE) activities in the cerebral synaptic membranes (SPM) and the peripheral red blood cells (RBC) of rats by in vivo and in vitro experiments. In the in vivo study, pregnant rats ingested ad libitum fluorinated drinking water (5, 15, 50 ppm F-) during their gestation and lactation. It was shown that the AChE activities of the SPM and peripheral RBCs in maternal rats exposed 5-50 ppm F- for 60 days were elevated significantly by 30.0-67.6% and 12.5-31.9% in a dose-dependent manner, respectively. The AChE activities of their offspring 80 days after birth were also increased (8.7-28.7% for SPM and 20.6-32.4% for RBC). In contrast, the AChE activities of SPM in vitro were inhibited by 5.0-50.0 mmol F-/L treatment in a time- and dose-dependent manner. Analysis with the Hanes plots suggested that the enzymesubstrate kinetics are consistent with a mixed type of inhibition.
本研究通过体内和体外实验观察氟化钠对大鼠脑突触膜（SPM）和外周红细胞（RBC）中乙酰胆碱酯酶（AChE）活性的影响。在体内研究中，怀孕大鼠在妊娠和哺乳期随意饮用氟化饮水（5、15、50 ppm F-）。结果表明，暴露于5 - 50 ppm F- 60天的母鼠SPM和外周RBC的AChE活性分别显著升高30.0 - 67.6%和12.5 - 31.9%，呈剂量依赖性。其后代出生80天后的AChE活性也增加（SPM为8.7 - 28.7%，RBC为20.6 - 32.4%）。相反，体外5.0 - 50.0 mmol F-/L处理以时间和剂量依赖性方式抑制SPM的AChE活性。用Hanes图分析表明酶 - 底物动力学符合混合型抑制。
