新生RNA与含PML核体中CREB结合蛋白的定位
Localization of nascent RNA and CREB binding protein with the PML-containing nuclear body.
作者信息
LaMorte V J, Dyck J A, Ochs R L, Evans R M
机构信息
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4991-6. doi: 10.1073/pnas.95.9.4991.
Abstract
The cellular role of the PML-containing nuclear bodies also known as ND10 or PODs remains elusive despite links to oncogenesis and viral replication. Although a potential role in transcription has been considered, direct evidence has been lacking. By developing a novel in vivo nucleic acid labeling approach, we demonstrate the existence of nascent RNA polymerase II transcripts within this nuclear body. In addition, PML and the transactivation cofactor, CREB binding protein (CBP), colocalize within the nucleus. Furthermore, we show that CBP in contrast to PML is distributed throughout the internal core of the structure. Collectively, these findings support a role for this nuclear body in transcriptional regulation.
摘要
含早幼粒细胞白血病（PML）的核体，也称为ND10或PODs，其细胞功能仍不清楚，尽管它与肿瘤发生和病毒复制有关。虽然曾考虑过其在转录过程中可能发挥的作用，但一直缺乏直接证据。通过开发一种新型的体内核酸标记方法，我们证明了该核体内存在新生的RNA聚合酶II转录本。此外，PML与反式激活辅因子——CREB结合蛋白（CBP）在细胞核内共定位。而且，我们发现与PML不同，CBP分布于该结构的整个内核。这些发现共同支持了该核体在转录调控中发挥作用。
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