Rodríguez-Belmonte E, González-Siso I, Cerdán E
Departamento de Biología Celular y Molecular, Universidad de La Coruña, Spain.
Curr Genet. 1998 Apr;33(4):262-7. doi: 10.1007/s002940050335.
The sequence and characterization of the KlGSK-3 gene in chromosome VI [corrected] from Kluyveromyces lactis is presented. The deduced amino-acid sequence predicts a protein of 415 amino acids and an Mr of 47 kDa. A computer search reveals significant homology to serine/threonine protein kinases closely related to members of the GSK-3 subfamily. The Klgsk-3::URA3 disrupted strain is unable to grow in glucose at 37 C but KlGSK-3 is not essential for vegetative growth at 30 C or 14 C. The KlGSK-3 gene presents the highest homology with the Saccharomyces cerevisiae MDS1 gene. Expression studies show an increase of mRNA levels caused both by carbon starvation and when diploids are shifted from rich to sporulation media. The data reported show that KlGSK-3, like MCK1 from S. cerevisiae, is related to glycogen storage.
本文介绍了乳酸克鲁维酵母菌第六条染色体上KlGSK-3基因[已修正]的序列及特征。推导的氨基酸序列预测该蛋白由415个氨基酸组成，分子量为47 kDa。计算机检索显示，它与与GSK-3亚家族成员密切相关的丝氨酸/苏氨酸蛋白激酶具有显著同源性。Klgsk-3::URA3基因敲除菌株在37℃下无法在葡萄糖中生长，但KlGSK-3对于30℃或14℃下的营养生长并非必需。KlGSK-3基因与酿酒酵母MDS1基因的同源性最高。表达研究表明，碳饥饿以及二倍体从丰富培养基转移到孢子形成培养基时，mRNA水平均会升高。报告的数据表明，KlGSK-3与酿酒酵母的MCK1一样，与糖原储存有关。
