Stevens D L, Bryant A E, Huffman J, Thompson K, Allen R C
Infectious Disease Section, Veterans Affairs Medical Center, Boise, Idaho.
J Infect Dis. 1994 Dec;170(6):1463-72. doi: 10.1093/infdis/170.6.1463.
The present study was undertaken to determine if measurement and analysis of phagocyte function are useful for diagnosis and staging of infection. Circulating phagocyte activity was measured in healthy volunteers and sequentially in patients with acute infections of different types and severity, including those with diabetes mellitus or human immunodeficiency virus (HIV) infection. Using an automated luminescence system, these phagocyte functions were measured in whole blood: basal and phorbol 12-myristate 13-acetate (PMA)-stimulated oxidase activity, basal and PMA-stimulated simple dioxygenation (e.g., oxidase-driven haloperoxidase activity), and circulating and primed opsonin receptor-dependent dioxygenation. Multiple discriminant analysis of these data showed significant differences between healthy controls, diabetic patients, HIV-positive subjects, and patients with pneumonia or sepsis syndromes. Longitudinally, circulating phagocyte function correlated with clinical condition, severity of infection, and outcome. This methodology provides rapid, objective, and sensitive diagnostic and monitoring information for patients with infections.
本研究旨在确定吞噬细胞功能的检测与分析是否有助于感染的诊断和分期。在健康志愿者中以及在患有不同类型和严重程度急性感染的患者（包括糖尿病患者或人类免疫缺陷病毒（HIV）感染者）中依次测量循环吞噬细胞活性。使用自动发光系统，在全血中测量这些吞噬细胞功能：基础和佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）刺激的氧化酶活性、基础和PMA刺激的简单双加氧作用（如氧化酶驱动的卤过氧化物酶活性）以及循环和致敏调理素受体依赖性双加氧作用。对这些数据进行的多判别分析显示，健康对照者、糖尿病患者、HIV阳性受试者以及患有肺炎或脓毒症综合征的患者之间存在显著差异。纵向来看，循环吞噬细胞功能与临床状况、感染严重程度及转归相关。该方法可为感染患者提供快速、客观且灵敏的诊断和监测信息。
