Chauhan A, Dilawari J B, Sharma R, Mukesh M, Saroa S R
Molecular Biology, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Vaccine. 1998 Apr;16(7):755-6. doi: 10.1016/s0264-410x(97)00252-1.
The role of long-persisting HEV antibodies in humans was investigated. A well-characterized human volunteer anti-HEV (IgG) after 4 years of the recovery of the disease was passively immunized (200 mg kg-1 body wt) intramuscularly into three rhesus monkeys (nos 7, 8 and 9), while two monkeys (nos 5 and 6) were injected with normal immunoglobulin preparation (negative for anti-HEV IgG). At 16 h later all the animals were challenged intravenously with live HEV, as 10% stool extract prepared from the volunteer (positive for HEV by solid-phase immune electron microscopy). It was seen that transaminases (ALT/AST) were elevated in immunized monkeys on day 49 (no. 8), 60 (no. 7) and 86 (no. 9) after the virus challenge. On serological examination, all immunized (nos 7, 8 and 9) and one unimmunized (no. 6) monkeys were found positive for HEV IgM on day 10 post-challenge, while monkey no. 5 was negative. Further, all the animals from the immunized and unimmunized group seroconverted to HEV-IgG when tested on days 25 and 55 post-challenge. This has clearly shown that there is no protection. Therefore, these long-persisting HEV antibodies alone in humans, may not be protective in passive immunization.
对人类中持续存在的戊型肝炎病毒（HEV）抗体的作用进行了研究。在一名疾病康复4年后具有明确特征的人类志愿者抗HEV（IgG），以200mg/kg体重的剂量通过肌肉注射被动免疫三只恒河猴（第7、8和9号），而另外两只猴子（第5和6号）注射正常免疫球蛋白制剂（抗HEV IgG阴性）。16小时后，所有动物静脉注射活的HEV，即从该志愿者粪便中制备的10%粪便提取物（通过固相免疫电子显微镜检测HEV呈阳性）。可以看到，在病毒攻击后第49天（第8号）、60天（第7号）和86天（第9号），免疫的猴子转氨酶（ALT/AST）升高。血清学检查发现，在攻击后第10天，所有免疫的（第7、8和9号）和一只未免疫的（第6号）猴子HEV IgM呈阳性，而第5号猴子为阴性。此外，在攻击后第25天和第55天进行检测时，免疫组和未免疫组的所有动物血清均转化为HEV-IgG。这清楚地表明没有保护作用。因此，人类中单独存在的这些持续存在的HEV抗体在被动免疫中可能没有保护作用。
