Park J H, Hyun C K, Shin H K
Institute of Functional Foods and Safety, Handong University, Pohang, Kyungbuk, South Korea.
Cancer Lett. 1998 Apr 10;126(1):43-8. doi: 10.1016/s0304-3835(97)00526-0.
The cytotoxic effects of preparations of Korean mistletoe (Viscum album L. var. coloratum Ohwi) on non-tumorigenic A31 cells and tumorigenic MSV cells were investigated. While the aqueous extract from Korean mistletoe (<8 microg/ml) showed strong cytotoxicity on both cell lines, the heat-treated extract was much less cytotoxic with TD50 values of above 300 microg/ml. The heat-treated extract showed a growth-enhancing effect on non-tumorigenic cells and a cytotoxic effect on tumorigenic cells. The alkaloids fraction, which was isolated from the crude extract, was not cytotoxic to non-tumorigenic A31 cells up to 550 microg/ml, but was cytotoxic to tumorigenic MSV cells at 138 microg/ml. Heat treatment did not change the cytotoxic effects of the alkaloids fraction, indicating that the selective cytotoxicity of the heat-treated mistletoe extract on tumorigenic MSV cells might be due to its alkaloids. In order to study the changes in the cytotoxicity of fermented Korean mistletoe, the crude and heat-treated extracts were inoculated with Lactobacillus plantarum. During 7 days of fermentation, the cytotoxicity of the fermented heat-treated extract was increased while that of the fermented crude extract was not changed significantly.
研究了韩国槲寄生（白果槲寄生变种 Ohwi）制剂对非致瘤性 A31 细胞和致瘤性 MSV 细胞的细胞毒性作用。韩国槲寄生的水提取物（<8 微克/毫升）对两种细胞系均表现出强细胞毒性，而热处理提取物的细胞毒性则小得多，其半数毒性剂量（TD50）值高于 300 微克/毫升。热处理提取物对非致瘤性细胞有生长促进作用，对致瘤性细胞有细胞毒性作用。从粗提取物中分离出的生物碱部分，在浓度高达 550 微克/毫升时对非致瘤性 A31 细胞无细胞毒性，但在浓度为 138 微克/毫升时对致瘤性 MSV 细胞有细胞毒性。热处理并未改变生物碱部分的细胞毒性作用，这表明热处理的槲寄生提取物对致瘤性 MSV 细胞的选择性细胞毒性可能归因于其生物碱。为了研究发酵韩国槲寄生细胞毒性的变化，将粗提取物和热处理提取物接种植物乳杆菌。在发酵的 7 天内，发酵热处理提取物的细胞毒性增加，而发酵粗提取物的细胞毒性没有明显变化。
